Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Phospho-MEK1 (Ser298) Detection Kit, 10,000 Assay Points
HTRF Human Phospho-MEK1 (Ser298) Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total CDK9 Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total CDK9 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human and Mouse Total IKZF1 Detection Kit, 500 Assay Points
HTRF Human and Mouse Total IKZF1 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human Total SMARCA-2 Detection Kit, 10,000 Assay Points
HTRF Human Total SMARCA-2 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Total FGFR4 Detection Kit, 10,000 Assay Points
HTRF Human Total FGFR4 Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Total HDAC5 Detection Kit, 500 Assay Points
HTRF Human & Mouse Total HDAC5 Detection Kit, 500 Assay Points
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-Cofilin (Ser3 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-Cofilin (Ser3 & Total) Detection Kit, 50,000 Assay Points
Molecular Devices Filter Kit, Standard, 7 Filters, 2 Slides
Replacement standard filter kit for the Molecular Devices SpectraMax iD5 multi-mode microplate reader. The kit provides a set of optical filters and slides used to configure the reader for common filter-based detection modes.
- Contains seven filters and two filter slides.
- Provides configurable filters for common fluorescence and filter-based detection modes.
- Supplied as a standard kit for instrument configuration and replacement of worn filters.
- Includes components intended for installation as instrument accessory slides and filter holders.
Molecular Devices Fluorescence Polarization Filter, 590 nm, 20 nm, Horizontal
This fluorescence polarization filter isolates emission at 590 nm with a 20 nm bandpass and is supplied with a holder for installation in compatible microplate readers, including SpectraMax models. It is intended for fluorescence polarization assays where precise emission isolation and polarized detection are required.
- Center wavelength 590 nm.
- Bandwidth 20 nm.
- Polarized orientation horizontal.
- Includes holder for instrument installation.
- Designed for fluorescence polarization assays.
- Compatible with polarization detection modules in microplate readers.